What DoD Policy governs the transmission and transportation of classified information?

The correct choice is DoD Manual 5200.01 because it specifically addresses the protection of classified information and outlines the policies and procedures related to the transmission and transportation of that information. This manual provides the necessary framework for safeguarding sensitive data and ensuring compliance with various regulations that govern the handling of classified materials. It establishes guidelines that all personnel must follow to protect national security interests and maintain the integrity of classified communications.

In contrast, the other choices may deal with related areas within the Department of Defense but do not focus specifically on the defined policies for the transmission and transportation of classified information. For example, DoD Directive 5240.01 mainly pertains to intelligence activities and oversight, while DoD Instruction 8500.01 focuses on cybersecurity and information assurance, and DoD Policy 3100.01 relates to operational capabilities and readiness, rather than the specifics of handling classified data transportation.